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2xstop buffer  (Qiagen)


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    Structured Review

    Qiagen 2xstop buffer
    2xstop Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2xstop+buffer/RNase+A/pm35523900-349-6-46
    Average 99 stars, based on 6790 article reviews
    2xstop buffer - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions.
    Article Snippet: To generate Mcm2-2A mutant cell lines, sgRNA targeting Mcm2 sites were cloned into the PX459 vector, and single-stranded oligo DNA nucleotides (ssODNs) were synthesized (IDT, Coralville, IA, USA) as the repair template. .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the AccelNGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using pairedend sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions
    Article Snippet: .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the Accel NGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using paired end sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Purification:

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions.
    Article Snippet: To generate Mcm2-2A mutant cell lines, sgRNA targeting Mcm2 sites were cloned into the PX459 vector, and single-stranded oligo DNA nucleotides (ssODNs) were synthesized (IDT, Coralville, IA, USA) as the repair template. .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the AccelNGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using pairedend sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions
    Article Snippet: .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the Accel NGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using paired end sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Polymerase Chain Reaction:

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions.
    Article Snippet: To generate Mcm2-2A mutant cell lines, sgRNA targeting Mcm2 sites were cloned into the PX459 vector, and single-stranded oligo DNA nucleotides (ssODNs) were synthesized (IDT, Coralville, IA, USA) as the repair template. .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the AccelNGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using pairedend sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions
    Article Snippet: .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the Accel NGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using paired end sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.

    Next-Generation Sequencing:

    Article Title: Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions
    Article Snippet: .. Reactions were stopped by mixing with 2XStop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% Digitonin, 25 μL 100 μg/ml RNase A) followed by incubation at 37 °C for 30 min. DNA in supernatant were purified using the QIAquick PCR Purification Kit (28104, Qiagen) and used for library preparation using the Accel NGS 1S Plus DNA library kit (Swift Bioscience, 10096). .. Each library DNAs were sequenced using paired end sequencing by Illumina NextSeq 500 platforms at the Columbia University Genome Center.



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